Work packages > Work package 5

Start date or starting event: Month 1
Work package title: Improvement in FMD diagnostics
Activity Type: RTD
Work Package Leader: IZSLER
Participants: CODA-VAR, IAH, CVI, IZSLER, FLI, AFSSA

Objectives

  • 1. Improve standardisation and harmonisation of FMD diagnostic results through the development of a panel of stabilised, validated diagnostic kits for FMD serology and antigen typing ready for commercial exploitation
  • 2. Develop and evaluate confirmatory DIVA tests and test systems for post-outbreak sero-surveillance (e.g. NSP-ELISA combinations, improved IgA ELISAs)
Develop and evaluate a multiplex immunoassay that allows simultaneous detection of antibodies to multiple viral antigens in a single reaction

Description of work

  • Task 1. Development of stabilised diagnostic kits
  • Task 2. Development of confirmatory DIVA tests and test systems
    •     2.1. Optimisation and validation of a polyclonal NSP ELISA (partner 3)
    •     2.2. Organisation of a validation workshop on confirmatory test systems including possible confirmatory tests
    •     2.3. Improvement of an ELISA to detect IgA in saliva of FMDV infected animals regardless of their vaccination status
  • Task 3. Development of a multiplex immunoassay

Deliverables

  • 5.1. Validated indirect IgA ELISA (M24)
  • 5.2. Stabilised prototype ELISA based kits for anti-FMDV structural protein antibody detection (serotypes O, A, Asia) (M36)
  • 5.3. Stabilised prototype ELISA based kits for FMDV antigen typing (all serotypes) (M36)
  • 5.4. Validated NSP-ELISA based on polyclonal sera (M36)
  • 5.5. Report of the workshop on confirmatory test systems including confirmatory tests (M36)
  • 5.6. Guidelines for the application of DIVA tests in case of post-outbreak sero-surveillance strategies following FMD vaccination, and for the detection of FMDV in FMD-free-with-vaccination regions (M36)
  • 5.7. Validated multiplex immunoassay (M36)
  • 5.8. Peer-reviewed publications (M36)

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